The Edge 200 was used to investigate the role of ER chaperones, and other heat shock protein family members, in the T cell changes that occur with the induction of type 1 diabetes. Cervical lymph nodes (CLN) were used from both control BBDR rats and BBDR rats treated to induce diabetes.

Changes in profiles of relative percentage distribution of several markers per fraction indicate their potential translocation in the cell.

The utility of Edge Technology and Centricollation has been demonstrated also in the study of ER stress induced diabetes using INS-1 pancreatic beta cells. ER stress-mediated beta-cell dysfunction and death have important functions in the pathogenesis of both type 1 and type 2 diabetes. IRE1 is a marker of ER Stress.

• The locations of the highest relative expressions of IRE1 change from the 40-50% sucrose fractions to the 15-30% sucrose fractions after treatment with thapsigargin. This finding indicates the potential trafficking of IRE1 in the cell.
• Density based fractionation provides valuable information connecting subcellular biology and biomarkers.