Centricollation process using Edge 200 Separation System, when combined with conventional bioanalytical techniques, such as western blot analysis, 2DE and mass spectrometry, provides a powerful and rapid method for identifying potential pathway biomarkers and their networks by:

• Centricollation-based subcellular fractionation using Edge 200 Separation System,
• probing of fractions using known pathway markers,
• determination of the fraction with the largest differential expression of the probed marker (i.e. diseased vs. control, diseased vs. treated, etc.), and
• identification of additional protein markers in that fraction which are related to the known markers through networks or pathways using 2DE and mass spectrometry.

Benefits:

• Sample size independent
• Lab to lab reproducibility
• Multiple marker discovery
• Discover subcellular specific pathways
• Determine subcellular translocation
• Identify regulatory mechanisms