The usefulness of Edge Technology and EDGE*TEST in evaluating markers of synaptic dysfunction was shown using rat models of high fat diet, since high fat diet has a profound impact on brain function and apoptosis risk.

• Phosphosynapsin I, a potential biomarker described in the literature, was evaluated as an indicator of synaptic dysfunction.
• Ratios of the phosphosynapsin marker expression between two specific fractions within a sample are different for control and HF diet samples, with relatively tight clustering of data within each sample group.
• The ratio of marker expression between two specific fractions can be used to determine if the sample is control or HF diet.